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Nexcelom Bioscience/Cell Fitness Panel Reagents/CSK-V0023-1
470/534 nm
470/534 nm
470/534 nm
Cell Fitness Panel Reagents
The Cell Fitness Panel Kit is designed for researchers interested in acquiring data on cell sample health and quality.
Many samples in cellular therapy, immunotherapy, and cell line development, are by their nature unique and precious. Patient-derived primary samples are often comprised of a heterogeneous cell population and display a wide range of physiological behaviors. Whether these samples underwent a freeze/thaw cycle, or cell isolation procedure, or have been cultured within a bioreactor for an extended period, the Cell Fitness Panel is used to assess not only the wellbeing of cell samples but also to offer potential insights into samples with low or crashing viabilities.
The Cell Fitness Panel consists of five individual tests to assess viability, vitality, mid and late-stage apoptosis, and reactive oxidative stress.
ViaStain™ Cell Fitness Panel
To determine cellular health, the kit contains five individual tests:
Viability
- More accurate than trypan blue
- No red blood cell (RBC) lysis needed
- Measure cell viability and concentration of nucleated cells in a single step
Kit provides Acridine orange / Propidium iodide
Vitality
- Identify the number of enzymatically active cells vs the number of dead cells in a population
- Rapid conversion from non-fluorescing calcein–AM to a brightly fluorescing signal in healthy cells
Kit provides Calcein AM/PI
Mid-stage Apoptosis
- Classic apoptosis marker, annexin V/PI, measures percent live, annexin V positive, and necrotic cells
- Optimized workflow for rapid staining, imaging, and data analysis
Kit provides Annexin V/PI
Late-stage Apoptosis
- Detection of an activated executioner protein Caspase 3/7 is a strong indicator poor cell health
- The bright green nuclear fluorescent signal is quickly and easily detected in unhealthy cells
Kit provides Caspase 3
Oxidative Stress
- Accumulation of reactive oxygen species (ROS) can result in cell damage and death
- The bright green fluorescent signal can be an early indication of an in trouble culture
Kit provides ROS-Total
Run Cell Fitness Panel tests on:
Cellometer Spectrum
Cellaca MX
| Description | Catalog Number | Unit Size | Excitation/Emission | Manual | SDS | |
|---|---|---|---|---|---|---|
| ViaStain™ Cell Fitness Panel for Cellometer Spectrum | CSK-V0023-1 | 25 tests per assay | 527/655 nm470/534 nm | US: Full KitEU: Full Kit | Buy Online | |
| ViaStain™ Cell Fitness Panel for Cellaca MX | CSK-V0024-1 | 1 Cellaca Plate per assay | 534/655 nm470/527 nm | US: Full KitEU: Full Kit | Buy Online |
Download the Cell Fitness Panel Assay Templates for Cellometer Spectrum »
Download the Cell Fitness Panel Assay Templates for Cellaca MX »
ViaStain™ Total ROS Green
Oxidative stress is caused by an accumulation of reactive oxygen species (ROS) within cells or tissues. While the production of ROS is part of the normal cellular metabolic processes, the cell’s inability to neutralize these radicals may have a direct impact on cell health, physiological functions, and possibly lead to cell damage or even cell death. Over the years, oxidative stress has been linked with multiple chronic and degenerative diseases such as cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, neurodegenerative diseases, and cancer. The role of antioxidants, including vitamins C and E, polyphenols, and flavonoids, in the reduction of oxidative stress, continues to be a major area of study.
ViaStain™ Total ROS Green kits for Cellometer Spectrum and Cellaca MX uses a unique ROS sensor to quantify ROS in live cells. ROS Green is a cell-permeable stain that generates a strong green fluorescence signal upon reaction with ROS to obtain quantitative cell metrics.
Features and Benefits:
- Validated for use on Cellometer Spectrum and Cellaca MX systems
- Rapid automatic data analysis using pre-existing templates
- Optimized easy-to-follow protocol for best results
Untreated, healthy, Jurkat cells (ROS negative, left) show very little green fluorescence while drug-treated cells (ROS positive, right) produce a bright green ROS positive signal.
| Description | Catalog Number | Unit Size | Excitation/Emission | Manual | SDS | |
|---|---|---|---|---|---|---|
| ViaStain™ Total ROS Green for Cellometer Spectrum | CSK-V0022-1 | 300 tests per assay | 470/534 nm | US: Full KitEU: Full Kit | Buy Online | |
| ViaStain™ Total ROS Green for Cellaca MX | CSK-V0019-1 | 10 Cellaca Plates per assay | 470/527 nm | US: Full KitEU: Full Kit | Buy Online |
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